Coding

Part:BBa_K331021:Design

Designed by: Adam Smith   Group: iGEM10_Lethbridge   (2010-09-26)


Catechol 2,3 Dioxygenase with Fusion Suffix


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 311
    Illegal NgoMIV site found at 483
    Illegal AgeI site found at 834
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chose to remove the promoter and endogenous ribosomal binding site (of BBa_J33204) so we could add a promoter of our choosing


Source

This part is the PCR product of BBa_K118021 using the following primers:

Forward: 5'-GAA TTC GCG GCC GCT TCT AGA TGA ACA AAG GTG TAA TGC GAC CG-3' (xylE Prefix Standard)

Reverse: 5'-CTG CAG CGG CCG CTA CTA GTG GTC AGC ACG GTC ATG AAT CG-3' (xylE Suffix Fusion)


References